hisC

histidinol-phosphate aminotransferase / tyrosine and phenylalanine aminotransferase

Locus: BSU_22620

Isoelectric point: 5.01

Molecular weight: 39.93 kDa

Protein length: 360 aa

Gene length: 1083 bp

Function: biosynthesis of aromatic amino acids

Product: histidinol-phosphate aminotransferase / tyrosine and phenylalanine aminotransferase

Essential: no

E.C.: 2.6.1.9

Synonyms: aroJ

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of aromatic amino acids

Gene

Coordinates: 2,370,415 2,371,497

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + L-histidinol phosphate --> 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate (according to UniProt)

Protein family

• class-II pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 3FFH (from Listeria innocua, 52% identity)

Expression and Regulation

Operons

• Genes: trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE
  Description: PubMed

  Regulatory mechanism

  • MtrB: termination/antitermination, PubMed, in MtrB regulon

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

• Genes: hisC-tyrA-aroE
  Description: PubMed

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• BKE22620 (hisC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCTTTGATACGCAAATCAG, downstream forward: _UP4_TAAGAGGTGATCAAATATCA
• BKK22620 (hisC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTCTTTGATACGCAAATCAG, downstream forward: _UP4_TAAGAGGTGATCAAATATCA

References

Reviews

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Original publications

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