SubtiBank SubtiBank
mtlD [2019-07-23 09:15:25]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

mtlD [2019-07-23 09:15:25]

mannitol-1-phosphate 5-dehydrogenase
Locus
BSU_03990
Isoelectric point
6.29
Molecular weight
40.31 kDa
Protein length
373 aa Sequence Blast
Gene length
1122 bp Sequence Blast
Function
mannitol utilization
Product
mannitol-1-phosphate 5-dehydrogenase
Essential
no
E.C.
1.1.1.17
Synonyms
mtlB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of mannitol

    • Gene

    Coordinates
    451,618 452,739

    The protein

    Catalyzed reaction/ biological activity

  • D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH (according to Swiss-Prot)

    • Protein family

  • mannitol dehydrogenase family (with UxaB, according to UniProt)

    • Structure

  • 3H2Z (from Shigella flexneri 2a str. 2457t mutant, 42% identity, 59% similarity)

    • Expression and Regulation

    Operons

    Genes
    mtlA-mtlF-mtlD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • MtlR: activation, PubMed, in MtlR regulon

    • Regulation

  • induced by mannitol (MtlR) PubMed

    • Additional information

  • An antisense RNA is predicted for mtlD PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C019 (mtlD::erm), available at the NBRP B. subtilis, Japan
  • BKE03990 (mtlD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGCACCGAAATGTAAGGCGA, downstream forward: _UP4_TAACCGACCACCCGTGACAC
  • BKK03990 (mtlD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CGCACCGAAATGTAAGGCGA, downstream forward: _UP4_TAACCGACCACCCGTGACAC

    • References

    Loading