yurI [2019-08-30 17:34:56]

ribonuclease, extracellular RNase Bsn

locus: BSU_32540

pI: 4.87

mw: 31.94 kDa

protein length: 288 aa

gene length: 867 bp

function: extracellular RNA degradation

product: extracellular RNase Bsn

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.3|Phosphate metabolism]
• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.2|Endoribonucleases]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 3,344,113 3,344,979

The protein

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|4D46B2842D6B8B8002F47544E76DB36C24364E69|yurI]
  description: [Pubmed|16291680]

  regulatory mechanism

  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|16291680], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|16291680]

Biological materials

Mutant

• MGNA-A552 (yurI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/552 NBRP B. subtilis, Japan]
• BKE32540 ([gene|4D46B2842D6B8B8002F47544E76DB36C24364E69|yurI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACTTGCCCTCCTTTTA, downstream forward: _UP4_TAAGAAAAAGGTGCTCCTTT
• BKK32540 ([gene|4D46B2842D6B8B8002F47544E76DB36C24364E69|yurI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACTTGCCCTCCTTTTA, downstream forward: _UP4_TAAGAAAAAGGTGCTCCTTT

References

• 16291680,18957862,12490701,1396690,20709850,20817675,25666134