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mrfA
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mrfA

ATP-dependent helicase, part of a novel nucleotide excision repair pathway
Locus
BSU_22220
Isoelectric point
6.16
Molecular weight
84.40 kDa
Protein length
749 aa Sequence Blast
Gene length
2250 bp Sequence Blast
Function
mitomycin C-specific DNA damage repair
Product
helicase
Essential
no
Synonyms
yprA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Genetics → DNA repair/ recombination → Other proteins

    • Gene

    Coordinates
    2,334,581 2,336,830

    Phenotypes of a mutant

  • sensitive to mitomycin C PubMed

    • The protein

    Protein family

  • helicase family (according to UniProt)

    • Domains

  • Helicase ATP-binding domain (aa 63-243) (according to UniProt)
  • Helicase C-terminal domain (aa 276-430) (according to UniProt)

    • Structure

  • 2P6R (the N-terminal domain, corresponds to aa 31 ... 451 of MrfA, 25% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    mrfA-mrfB-cotD
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-A412 (yprA::erm), available at the NBRP B. subtilis, Japan
  • BKE22220 (mrfA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTTTACACCTCTTTGT, downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC
  • BKK22220 (mrfA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTTTACACCTCTTTGT, downstream forward: _UP4_ATGTCGTAAGGAGGGAGGCC

    • References

    Research papers

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