SubtiBank SubtiBank
trpD
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trpD

anthranilate phosphoribosyltransferase
Locus
BSU_22670
Isoelectric point
4.90
Molecular weight
35.87 kDa
Protein length
338 aa Sequence Blast
Gene length
1017 bp Sequence Blast
Function
biosynthesis of tryptophan
Product
anthranilate phosphoribosyltransferase
Essential
no
E.C.
2.4.2.18
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,374,881 2,375,897

The protein

Catalyzed reaction/ biological activity

  • diphosphate + N-(5-phospho-β-D-ribosyl)anthranilate --> 5-phospho-α-D-ribose 1-diphosphate + anthranilate (according to UniProt)
  • Protein family

  • anthranilate phosphoribosyltransferase family (single member, according to UniProt)
  • Structure

  • 4YI7 (from Acinetobacter sp., 42% identity)
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • MtrB: termination/antitermination, PubMed, in MtrB regulon
  • Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the RNA switch RNA is degraded by RNase Y PubMed
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    Description

    Regulatory mechanism

  • MtrB: termination/antitermination, PubMed, in MtrB regulon
  • Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
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    Biological materials

    Mutant

  • BKE22670 (trpD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTCACCGGCAGTAAGGGTTT, downstream forward: _UP4_CTAAAGCAGAAAGAGGAAGA
  • BKK22670 (trpD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTCACCGGCAGTAAGGGTTT, downstream forward: _UP4_CTAAAGCAGAAAGAGGAAGA
  • References

    Reviews

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    Original publications

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