nrnA [2017-09-16 17:28:10]

oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase

locus: BSU29250

pI: 4.67

mw: 34.93 kDa

protein length: 313 aa

gene length: 939 bp

function: degradation of RNA oligonucleotides

product: oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase

essential: no

ec: 3.1.3.7

synonyms: ytqI

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.1|Exoribonucleases]

Gene

Coordinates: 2,995,908 → 2,996,849

Phenotypes of a mutant

• The mutant requires cysteine [Pubmed|17586819]
• inactivation of ''[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]'' reduces sporulation efficiency to 5% that of wild type cells; delayed entry into sporulation, reduced SigmaG activity, and production of small forespores [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• oligoribonuclease (nanoRNAse), 3',5'-bisphosphate nucleotidase [Pubmed|17586819]
• hydrolyzes oligo(deoxy)ribonucleotides in a 5'→ 3' direction [Pubmed|28894100,21087930]
• hydrolyzes nano-RNAs in a 3'→ 5' direction [Pubmed|28894100]

[SW|Domains]

• [SW|DHH-DHHA1 domain]

Structure

• [PDB|5J21] [pubmed|28894100]

[SW|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operons

• genes: [gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]
  description: [Pubmed|9387221]

Biological materials

Mutant

• MGNA-A151 (ytqI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/151 NBRP B. subtilis, Japan]
• GP2155 (''[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
• BKE29250 (Δ[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]::erm trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU29250 BGSC], upstream reverse:

  _UP1_CATTTGATACTCCTTTATTG

  , downstream forward:

  _UP4_GAGTAGAGGGGAGACCCTCT

• BKK29250 (Δ[gene|4C630B61249F8F2369E9BFE8A49885D3225DE7B6|nrnA]::kan trpC2), available at [http://www.bgsc.org/getdetail.php?bgscid=BSU29250 BGSC], upstream reverse:

  _UP1_CATTTGATACTCCTTTATTG

  , downstream forward:

  _UP4_GAGTAGAGGGGAGACCCTCT

References

• 17586819,9387221,16479537,19429620,19553197,21087930,22102036,26691161,21768284,24013631,26735940,27834680,28894100