nrnA [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase

locus: BSU29250

pI: 4.00

mw: 34.00 kDa

function: degradation of RNA oligonucleotides

product: oligoribonuclease (nanoRNase), 3,5-bisphosphate nucleotidase

essential: no

ec: 3.1.3.7

synonyms: ytqI

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.1|Exoribonucleases]

[SW|Categories] containing this gene/protein

• [SW|RNAses], [SW|sporulation/ other]

Gene

Coordinates on the chromosome (coding sequence): 2,995,908 -> 2,996,849

Phenotypes of a mutant

• The mutant requires cysteine [Pubmed|17586819]
• inactivation of ''[SW|nrnA]'' reduces sporulation efficiency to 5% that of wild type cells; delayed entry into sporulation, reduced SigmaG activity, and production of small forespores [Pubmed|26735940]

The protein

Catalyzed reaction/ biological activity

• Adenosine 3',5'-bisphosphate H₂O = adenosine 5'-phosphate phosphate (according to Swiss-Prot)
• oligoribonuclease (nanoRNAse), 3',5'-bisphosphate nucleotidase [Pubmed|17586819]
• hydrolyze oligo(deoxy)ribonucleotides in a 5'-3' direction [Pubmed|21087930]

Structure

• [PDB|3DEV] (from S. haemolyticus, 49% identity)

[SW|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

Expression and Regulation

Operon

• ''[protein|search|nrnA]'' [Pubmed|9387221]

Biological materials

Mutant

• GP2155 (''[protein|search|nrnA]''::''aphA3''), available in [SW|Jörg Stülke]'s lab

References

• 17586819,9387221,16479537,19429620,19553197,21087930,22102036,26691161,21768284,24013631,26735940,27834680