cca [Mon Aug 31 2015 13:55:16 GMT+0200 (CEST)]

tRNA nucleotidyltransferase, maturation of the single-copy tRNACys, which lacks an encoded CCA 3 end

locus: BSU22450

pI: 8.04

mw: 45.00 kDa

function: tRNA modification

product: tRNA nucleotidyltransferase

essential: yes

ec: 2.7.7.25

synonyms: papS, ypjI

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

syns: papS, ypjI

[SW|Categories] containing this gene/protein

• [SW|translation], [SW|essential genes]

This gene is a member of the following [SW|regulons]

• [SW|Spx regulon]

Gene

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + tRNA(n) = diphosphate + tRNA(n+1) (according to Swiss-Prot)

Protein family

• the protein is similar to the ''E. coli'' poly(A) polymerase

Structure

• [PDB|1MIV] (Geobacillus stearothermophilus 44% identity)

Expression and Regulation

Operon

• ''[protein|search|ypjD]-[protein|search|dapB]-[protein|search|mgsA]-[SW|bshB1]-[protein|search|bshA]-[protein|search|cca]-[protein|search|birA]'' [Pubmed|20308541]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|23894131]

Regulation

• induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]

Regulatory mechanism

• [protein|search|Spx]: transcription activation [Pubmed|23894131]

References

Reviews

• 19883645,16364630,20155482,18523015

Original publications

• 17179213,12526808,20360175,9829937,16109934

Operon and expression

• 20308541,23894131