yrhJ [2019-09-05 10:39:10]

cytochrome P450 (CYP102A3)/ NADPH-cytochrome P450 reductase

locus: BSU_27160

pI: 6.02

mw: 118.47 kDa

protein length: 1054 aa

gene length: 3165 bp

function: fatty acid metabolism

product: NADPH-cytochrome P450 reductase

essential: no

ec: 1.6.2.4

synonyms: cypB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]
• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

Gene

Coordinates: 2,773,890 2,777,054

The protein

Catalyzed reaction/ biological activity

• hydroxylates medium-chain fatty acids in subterminal positions [Pubmed|14741768]
• organic molecule + O₂ + reduced [NADPH—hemoprotein reductase] --> alcohol + H⁺ + H₂O + oxidized [NADPH—hemoprotein reductase] (according to UniProt)
• NADPH + 2 oxidized [cytochrome P450] --> H⁺ + NADP⁺ + 2 reduced [cytochrome P450] (according to UniProt)

Protein family

• N-terminal part: [SW|cytochrome P450] family (according to UniProt)

Paralogous protein(s)

• [protein|9AA8CAEB42B1ED4CC8D80D57B045A55307114D6A|YetO]

Structure

• [PDB|2X7Y] (from Bacillus megaterium; 67% identity, 88% similarity) [Pubmed|25325618,22949185]

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]-[gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]
  description: [Pubmed|10917605]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|18179421,12775685], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|12207695], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|9636707], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulatory mechanism

  • [protein|3DA3FFB295F5513A220CB163994EE0F356C810E6|FatR]: repression, [Pubmed|11734890], in [regulon|3DA3FFB295F5513A220CB163994EE0F356C810E6|FatR regulon]

  regulation

  • expression is reduced in a [protein|search|SigV] mutant [Pubmed|21926231]

  additional information

  • A [protein|search|ncRNA] ([SW|RnaC]) is encoded between '[protein|search|yrhK]' and '[protein|search|yrhJ]' [PubMed|25790031]

• genes: [gene|6D5089931FBDB398A7FAF3C8DDF341EB4949AEFE|yrhH]-[gene|3DA3FFB295F5513A220CB163994EE0F356C810E6|fatR]-[gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]
  description: [Pubmed|18179421]

  regulation

  • Expressed under stress conditions ([protein|search|SigW], [protein|search|SigX]) [Pubmed|9683469]

  additional information

  • A [protein|search|ncRNA] ([SW|RnaC]) is encoded between '[protein|search|yrhK]' and '[protein|search|yrhJ]' [PubMed|25790031]

Biological materials

Mutant

• MGNA-A163 (yrhJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/163 NBRP B. subtilis, Japan]
• BKE27160 ([gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGTTTCATTAACATCTCCC, downstream forward: _UP4_TAAAATATAAAATCCCGCCA
• BKK27160 ([gene|4BA5214C441C6671CB7DAA3CACBCA5FC025BE0E6|yrhJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGTTTCATTAACATCTCCC, downstream forward: _UP4_TAAAATATAAAATCCCGCCA

References

• 11734890,10917605,11574077,14741768,15122913,12775685,16716428,16381045,20525796,12207695,9636707,21926231,25325618,22949185,25790031