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yrhJ [2019-09-05 10:48:11]
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yrhJ [2019-09-05 10:48:11]

cytochrome P450 (CYP102A3)/ NADPH-cytochrome P450 reductase
Locus
BSU_27160
Isoelectric point
6.02
Molecular weight
118.47 kDa
Protein length
1054 aa Sequence Blast
Gene length
3165 bp Sequence Blast
Function
fatty acid metabolism
Product
NADPH-cytochrome P450 reductase
Essential
no
E.C.
1.6.2.4
Synonyms
cypB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Electron transport and ATP synthesis → Electron transport/ other
  • Metabolism → Lipid metabolism → Lipid metabolism/ other
  • Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)

    • Gene

    Coordinates
    2,773,890 2,777,054

    The protein

    Catalyzed reaction/ biological activity

  • hydroxylates medium-chain fatty acids in subterminal positions PubMed
  • organic molecule + O2 + reduced [NADPH—hemoprotein reductase] --> alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase] (according to UniProt)
  • NADPH + 2 oxidized [cytochrome P450] --> H+ + NADP+ + 2 reduced [cytochrome P450] (according to UniProt)

    • Protein family

  • N-terminal part: cytochrome P450 family (according to UniProt)

    • Paralogous protein(s)

  • YetO

    • Domains

  • Flavodoxin-like domain (aa 486-625) (according to UniProt)
  • FAD-binding FR-type domain (aa 663-896) (according to UniProt)

    • Structure

  • 2X7Y (from Bacillus megaterium; 67% identity, 88% similarity) PubMed

    • Localization

  • cytoplasm (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    fatR-yrhJ
    Description
    PubMed

    Sigma factors

  • SigM: sigma factor, PubMed, in SigM regulon
  • SigW: sigma factor, PubMed, in SigW regulon
  • SigX: sigma factor, PubMed, in SigX regulon

    • Regulatory mechanism

  • FatR: repression, PubMed, in FatR regulon

    • Regulation

  • expression is reduced in a SigV mutant PubMed

    • Additional information

  • A ncRNA (RnaC) is encoded between 'yrhK' and 'yrhJ' PubMed
    • view in new tab

    Genes
    yrhH-fatR-yrhJ
    Description
    PubMed

    Regulation

  • Expressed under stress conditions (SigW, SigX) PubMed

    • Additional information

  • A ncRNA (RnaC) is encoded between 'yrhK' and 'yrhJ' PubMed
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A163 (yrhJ::erm), available at the NBRP B. subtilis, Japan
  • BKE27160 (yrhJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGTTTCATTAACATCTCCC, downstream forward: _UP4_TAAAATATAAAATCCCGCCA
  • BKK27160 (yrhJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGTTTCATTAACATCTCCC, downstream forward: _UP4_TAAAATATAAAATCCCGCCA

    • References

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