purF [2018-01-03 11:56:29]

glutamine phosphoribosyldiphosphate amidotransferase

Locus: BSU06490

Isoelectric point: 5.87

Molecular weight: 51.45 kDa

Protein length: 476 aa

Gene length: 1428 bp

Function: purine biosynthesis

Product: glutamine phosphoribosyldiphosphate amidotransferase

Essential: no

E.C.: 2.4.2.14

Synonyms: purB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides

Gene

Coordinates: 705,441 → 706,871

The protein

Catalyzed reaction/ biological activity

• 5-phospho-beta-D-ribosylamine diphosphate L-glutamate = L-glutamine 5-phospho-alpha-D-ribose 1-diphosphate H₂O (according to Swiss-Prot)

Structure

• 1GPH, 1AO0 (complex with ADP and GMP)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • G-box: termination, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon

  Regulation

  • expression activated by glucose (4.4 fold) PubMed

Biological materials

Mutant

• BKE06490 (ΔpurF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCCAAAAACGCCGCATTCTT, downstream forward: _UP4_TAAAACTTGAAAAATGACAT
• BKK06490 (ΔpurF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCCAAAAACGCCGCATTCTT, downstream forward: _UP4_TAAAACTTGAAAAATGACAT

References

Reviews

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Original publications

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