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thrC [2019-02-04 16:22:19]
function
biosynthesis of threonine
product
threonine synthase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine]Gene
Coordinates
3,313,770 3,314,828
The protein
Catalyzed reaction/ biological activity
O-phospho-L-homoserine + H2O --> L-threonine + phosphate (according to UniProt)has additional threonine dehydratase activity [pubmed|27260660]Protein family
threonine synthase family (according to UniProt)[SW|Cofactors]
PLPStructure
[PDB|1UIN] (from ''Thermus thermophilus'', 51% identity, 69% similarity) [Pubmed|12952961]additional information
belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]Expression and Regulation
Operons
genes
[gene|0F40291BB10DB6D7DFD94B9C7D700F4410C4EE69|yutH]-[gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]
description
[Pubmed|22383849]
regulation
expressed during [SW|sporulation] [Pubmed|22383849]view in new tabgenes
[gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]
description
[Pubmed|12107147]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|24163341], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon][protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|25755103], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon][protein|A4B7CF7A1C704C750AFAD97A43AF975260935083|ThrR]: repression, [Pubmed|27260660], in [regulon|A4B7CF7A1C704C750AFAD97A43AF975260935083|ThrR regulon]regulation
expressed in the presence of lysine or cysteine ([SW|ThrR]) [Pubmed|27260660]view in new tabBiological materials
Mutant
GP3030 (''[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]''::''spec''), available in [SW|Jörg Stülke]'s lab1A773 ( ''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [SW|Jrg Stlke]'s labBKE32250 ([gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCGBKK32250 ([gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCGReferences
12107147,3098560,24163341,15378759,27260660