thrC [2019-01-28 10:48:23]

threonine synthase

Locus: BSU32250

Isoelectric point: 5.18

Molecular weight: 37.31 kDa

Protein length: 352 aa

Gene length: 1056 bp

Function: biosynthesis of threonine

Product: threonine synthase

Essential: no

E.C.: 4.2.3.1

Synonyms: thrB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of lysine/ threonine

Gene

Coordinates: 3,313,770 → 3,314,828

The protein

Catalyzed reaction/ biological activity

• O-phospho-L-homoserine + H₂O = L-threonine + phosphate (according to Swiss-Prot)

Protein family

• serine/threonine dehydratase family (according to Swiss-Prot)

Structure

• 1UIN (from Thermus thermophilus, 51% identity, 69% similarity) PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: yutH-hom-thrC-thrB
  Description: PubMed

  Regulation

  • expressed during sporulation PubMed

• Genes: hom-thrC-thrB
  Description: PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
  • TnrA: repression, PubMed, in TnrA regulon
  • ThrR: repression, PubMed, in ThrR regulon

  Regulation

  • expressed in the presence of lysine or cysteine (ThrR) PubMed

Biological materials

Mutant

• 1A773 ( thrC::cat), PubMed, available at BGSC and in Jörg Stülke's lab
• BKE32250 (ΔthrC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
• BKK32250 (ΔthrC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG

References

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