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thrC [2019-01-28 10:48:23]
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thrC [2019-01-28 10:48:23]

threonine synthase
Locus
BSU32250
Isoelectric point
5.18
Molecular weight
37.31 kDa
Protein length
352 aa Sequence Blast
Gene length
1056 bp Sequence Blast
Function
biosynthesis of threonine
Product
threonine synthase
Essential
no
E.C.
4.2.3.1
Synonyms
thrB

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,313,770 → 3,314,828

The protein

Catalyzed reaction/ biological activity

  • O-phospho-L-homoserine + H2O = L-threonine + phosphate (according to Swiss-Prot)
  • Protein family

  • serine/threonine dehydratase family (according to Swiss-Prot)
  • Structure

  • 1UIN (from Thermus thermophilus, 51% identity, 69% similarity) PubMed
  • Additional information

  • belongs to the 100 most abundant proteins PubMed
  • Expression and Regulation

    Operons

    Description

    Regulation

  • expressed during sporulation PubMed
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    Description

    Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
  • TnrA: repression, PubMed, in TnrA regulon
  • ThrR: repression, PubMed, in ThrR regulon
  • Regulation

  • expressed in the presence of lysine or cysteine (ThrR) PubMed
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    Biological materials

    Mutant

  • 1A773 ( thrC::cat), PubMed, available at BGSC and in Jörg Stülke's lab
  • BKE32250 (ΔthrC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
  • BKK32250 (ΔthrC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
  • References

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