thrC

threonine synthase, has a minor threonine dehydratase (IlvA) activity

Locus: BSU_32250

Isoelectric point: 5.18

Molecular weight: 37.31 kDa

Protein length: 352 aa

Gene length: 1059 bp

Function: biosynthesis of threonine

Product: threonine synthase

Essential: no

E.C.: 4.2.3.1

Synonyms: thrB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of lysine/ threonine

Gene

Coordinates: 3,313,770 3,314,828

Phenotypes of a mutant

• auxotrophic for threonine PubMed

The protein

Catalyzed reaction/ biological activity

• O-phospho-L-homoserine + H₂O --> L-threonine + phosphate (according to UniProt)
• has additional threonine dehydratase activity PubMed

Protein family

• threonine synthase family (single member, according to UniProt)

Cofactors

• PLP

Structure

• 6CGQ (complexed with PLP and PLP-Ala) PubMed
• 6NMX (complexed with PLP and (Z)-L-2-amino-5-phosphono-3-pentenoic acid (APPA)) PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: yutH-hom-thrC-thrB
  Description: PubMed

  Regulation

  • expressed during sporulation PubMed

• Genes: hom-thrC-thrB
  Description: PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in CodY regulon
  • TnrA: repression, PubMed, in TnrA regulon
  • ThrR: repression, PubMed, in ThrR regulon

  Regulation

  • expressed in the presence of lysine or cysteine (ThrR) PubMed

Biological materials

Mutant

• GP3030 ΔthrC::spc, available in Jörg Stülke's lab PubMed
• 1A773 (thrC::cat), PubMed, available at BGSC and in Jörg Stülke's lab
• BKE32250 (thrC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
• BKK32250 (thrC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG

References

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