thrC

threonine synthase, has a minor threonine dehydratase ([protein|D0CF32BF81AA1DC7BBBC3E9B667A54F97260DF3F|IlvA]) activity

locus: BSU_32250

pI: 5.18

mw: 37.31 kDa

protein length: 352 aa

gene length: 1059 bp

function: biosynthesis of threonine

product: threonine synthase

essential: no

ec: 4.2.3.1

synonyms: thrB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.6|Biosynthesis/ acquisition of lysine/ threonine]

Gene

Coordinates: 3,313,770 3,314,828

Phenotypes of a mutant

• auxotrophic for threonine [pubmed|32743959]

The protein

Catalyzed reaction/ biological activity

• O-phospho-L-homoserine + H₂O --> L-threonine + phosphate (according to UniProt)
• has additional threonine dehydratase activity [pubmed|27260660]

Protein family

• threonine synthase family (single member, according to UniProt)

[SW|Cofactors]

• PLP

Structure

• [PDB|6CGQ] (complexed with PLP and PLP-Ala) [pubmed|30830751]
• [PDB|6NMX] (complexed with PLP and (Z)-L-2-amino-5-phosphono-3-pentenoic acid (APPA)) [Pubmed|30830751]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|0F40291BB10DB6D7DFD94B9C7D700F4410C4EE69|yutH]-[gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]
  description: [Pubmed|22383849]

  regulation

  • expressed during [SW|sporulation] [Pubmed|22383849]

• genes: [gene|11421D5BD3AB6C205DDA0864F878E2B04340033F|hom]-[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]-[gene|290302BA8A4E44C62AE3071C1F7DE18B20605607|thrB]
  description: [Pubmed|12107147]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|24163341], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|25755103], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|A4B7CF7A1C704C750AFAD97A43AF975260935083|ThrR]: repression, [Pubmed|27260660], in [regulon|A4B7CF7A1C704C750AFAD97A43AF975260935083|ThrR regulon]

  regulation

  • expressed in the presence of lysine or cysteine ([SW|ThrR]) [Pubmed|27260660]

Biological materials

Mutant

• GP3030 Δ[gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]::''spc'', available in [SW|Jörg Stülke]'s lab [pubmed|32743959]
• 1A773 (''thrC''::''cat''), [Pubmed|8973347], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A773&Search=1A773 BGSC] and in [SW|Jörg Stülke]'s lab
• BKE32250 ([gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG
• BKK32250 ([gene|4A839F8A53DF75DF01FE410EBD3361759C3B1C86|thrC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32250 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGATGGATAAGTCCTTTCC, downstream forward: _UP4_TATGTAAAAGGAGCGGCCCG

References

• 12107147,3098560,24163341,15378759,27260660,30830751,4630852,4199513,32743959