iolT

major transporter for inositol

Locus: BSU_06230

Isoelectric point: 9.57

Molecular weight: 51.47 kDa

Protein length: 473 aa

Gene length: 1422 bp

Function: myo-inositol uptake

Product: major transporter for inositol

Essential: no

Synonyms: ydjK

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Carbohydrate transporter
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of inositol
• Groups of genes → Membrane proteins

Gene

Coordinates: 676,442 677,863

The protein

Catalyzed reaction/ biological activity

• transport of myo-inositol PubMed

Protein family

• major facilitator superfamily (according to UniProt)
• Sugar transporter (TC 2.A.1.1) family (according to UniProt)

Paralogous protein(s)

• IolT, AraE, YwtG, YfiG, YncC, CsbC, IolT

Structure

• 4LDS (glucose transporter from Staphylococcus epidermidis, 36% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: iolT
  Description: PubMed

  Regulatory mechanism

  • IolR: repression, PubMed, in IolR regulon

  Regulation

  • induced in the presence of inositol (IolR) PubMed

Biological materials

Mutant

• MGNA-C218 (ydjK::erm), available at the NBRP B. subtilis, Japan
• BKE06230 (iolT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTGTTTCCCCCAGTCT, downstream forward: _UP4_TAATTTAAAAAAGAATCCGC
• BKK06230 (iolT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACTGTTTCCCCCAGTCT, downstream forward: _UP4_TAATTTAAAAAAGAATCCGC

References

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