cwlJ

spore coat protein, cell wall hydrolase

Locus: BSU_02600

Isoelectric point: 9.35

Molecular weight: 16.22 kDa

Protein length: 142 aa

Gene length: 429 bp

Function: spore germination

Product: spore coat protein, cell wall hydrolase

Essential: no

Synonyms: ycbQ

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall/ other
• Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class III
• Lifestyles → Sporulation → Germination → Additional germination proteins

Gene

Coordinates: 282,469 282,897

The protein

Protein family

• cwlJ family (single member, according to UniProt)

Effectors of protein activity

• activity depends on SwsB PubMed

Localization

• outer edge of the spore cortex PubMed
• inner spore coat, localization depends on SafA, CotE, and GerQ PubMed

Expression and Regulation

Operons

• Genes: cwlJ
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon
  • SigK: sigma factor, PubMed, in SigK regulon

  Regulatory mechanism

  • SpoIIID: activation, PubMed, in SpoIIID regulon

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, SpoIIID) PubMed
  • strongly repressed in the presence of salt (1.2 M NaCl) PubMed

Biological materials

Mutant

• 1G19 ( cwlJ::tet), PubMed, available at BGSC
• BKE02600 (cwlJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCACGTCACCTCCCCTTG, downstream forward: _UP4_TAGAGACAGGACACCGTTCA
• BKK02600 (cwlJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCACGTCACCTCCCCTTG, downstream forward: _UP4_TAGAGACAGGACACCGTTCA

References

Reviews

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Original publications

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