liaR [2019-03-12 10:26:36]

two-component response regulator, regulation of the liaI-liaH-liaG-liaF-liaS-liaR operon in response to bacitracin

Locus: BSU33080

Isoelectric point: 4.96

Molecular weight: 22.98 kDa

Protein length: 211 aa

Gene length: 636 bp

Function: regulation of the liaI-liaH-liaG-liaF-liaS-liaR operon

Product: two-component response regulator

Essential: no

Synonyms: yvqC

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
• Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 3,394,422 3,395,057

The protein

Catalyzed reaction/ biological activity

• regulation of the liaI-liaH-liaG-liaF-liaS-liaR operon in response to bacitracin

Paralogous protein(s)

• YhcZ, YdfI, YxjL, LnrK

Modification

• phosphorylation on a Asp residue by LiaS

Structure

• 5HEV (LiaR from Enterococcus faecium, 62% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: liaG-liaF-liaS-liaR
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • LiaR: activation, PubMed, in LiaR regulon

  Regulation

  • liaG: constitutive

• Genes: liaI-liaH-liaG-liaF-liaS-liaR
  Description: PubMed

  Regulation

  • liaG: constitutive

Biological materials

Mutant

• MGNA-B034 (yvqC::erm), available at the NBRP B. subtilis, Japan
• BKE33080 (liaR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCCCATTCTGACCATTTCAT, downstream forward: _UP4_AATCATCTCGTGAATTAGGC
• BKK33080 (liaR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCCCATTCTGACCATTTCAT, downstream forward: _UP4_AATCATCTCGTGAATTAGGC

Labs working on this gene/protein

• John Helmann, Cornell University, USA Homepage

References

Reviews

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Original publications

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