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maf
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maf

nucleotide pyrophosphatase
Locus
BSU_28050
Isoelectric point
5.71
Molecular weight
21.15 kDa
Protein length
189 aa Sequence Blast
Gene length
570 bp Sequence Blast
Function
unknown
Product
nucleotide pyrophosphatase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,862,572 2,863,141

The protein

Catalyzed reaction/ biological activity

  • nucleotide pyrophosphatase PubMed
  • dTTP + H2O --> diphosphate + dTMP + H+ (according to UniPort)
  • H2O + UTP --> diphosphate + H+ + UMP (according to UniPort)
  • CTP + H2O --> CMP + diphosphate + H+ (according to UniPort)
  • H2O + ψ-UTP --> diphosphate + H+ + ψ-UMP (according to UniPort)
  • 5-methyl-CTP + H2O --> 5-methyl-CMP + diphosphate + H+ (according to UniPort)
  • 5-methyl-UTP + H2O --> 5-methyl-UMP + diphosphate + H+ (according to UniPort)
  • Protein family

  • maf family (single member, according to UniProt)
  • Cofactors

  • binds phosphorylated compounds (UTP, IMP, IDP, GDP and others) in in vitro experiments
  • Structure

    Localization

  • cytosolic protein PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigM: sigma factor, promoter p2 within Maf PubMed, in SigM regulon
  • SigA: sigma factor, promoter p1, upstream of Maf PubMed, in SigA regulon
  • Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • Regulation

  • constitutively expressed PubMed
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    Biological materials

    Mutant

  • BKE28050 (maf::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCGTCATCCTTTCAG, downstream forward: _UP4_TGACGGTTCTATAAACGGGA
  • References

    Original publications

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