similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase, 6th step in the MEP pathway of isoprenoid biosynthesis
function
MEP pathway of isoprenoid biosynthesis
product
1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][SW|Categories] containing this gene/protein
[SW|biosynthesis of lipids], [SW|sporulation proteins], [SW|essential genes]This gene is a member of the following [SW|regulons]
[SW|SigE regulon]Gene
Coordinates on the chromosome (coding sequence)
2,589,123 -> 2,590,256
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate H2O protein-disulfide = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate protein-dithiol (according to Swiss-Prot) Protein family
ispG family (according to Swiss-Prot)[SW|Cofactors]
contains an iron-sulfur clusterStructure
[PDB|3NOY] (from ''Aquifex aeolicus'', 51% identity) [Pubmed|20932974][SW|Localization]
secreted (according to Swiss-Prot)Expression and Regulation
Operon
''[protein|search|yqfZ]-[protein|search|yqfY]'' [Pubmed|12662922][SW|Sigma factor]
[protein|search|SigE] [Pubmed|12662922]Regulation
expressed early during sporulation in the mother cell ([protein|search|SigE], [SW|SpoIIID]) [Pubmed|12662922,15383836]Regulatory mechanism
[SW|SpoIIID]: transcription repression [Pubmed|15383836]References
12662922,15383836,17458547,23832336,23840410,20932974 
