ispG

similar to peptidoglycan acetylation, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase, 6th step in the MEP pathway of isoprenoid biosynthesis

Locus: BSU_25070

Isoelectric point: 6.80

Molecular weight: 40.42 kDa

Protein length: 377 aa

Gene length: 1134 bp

Function: MEP pathway of isoprenoid biosynthesis

Product: 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase

Essential: yes

E.C.: 1.17.7.3

Synonyms: yqfY

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of isoprenoids
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Groups of genes → Essential genes

Gene

Coordinates: 2,589,123 2,590,256

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• (2E)-4-hydroxy-3-methylbut-2-enyl diphosphate + 2 H⁺ + H₂O + oxidized [flavodoxin] --> 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + reduced [flavodoxin] (according to UniProt)

Protein family

• ispG family (single member, according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 3NOY (from Aquifex aeolicus, 51% identity) PubMed

Localization

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yqfZ-ispG
  Description: PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in SigE regulon

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in SpoIIID regulon

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

References

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