trpE

anthranilate synthase (subunit I)

Locus: BSU_22680

Isoelectric point: 5.25

Molecular weight: 57.95 kDa

Protein length: 515 aa

Gene length: 1548 bp

Function: biosynthesis of tryptophan

Product: anthranilate synthase (subunit I)

Essential: no

E.C.: 4.1.3.27

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of aromatic amino acids

Gene

Coordinates: 2,375,869 2,377,416

The protein

Catalyzed reaction/ biological activity

• chorismate + L-glutamine --> anthranilate + H⁺ + L-glutamate + pyruvate (according to UniProt)

Protein family

• Anthranilate synthase component I family (with PabB, according to UniProt)

Effectors of protein activity

• subject to feedback inhibtion by tryptophan PubMed

Structure

• 1I7Q (from Serratia marcescens, 42% identity, 62% similarity) PubMed

Expression and Regulation

Operons

• Genes: trpE-trpD-trpC-trpF-trpB-trpA
  Description: PubMed

  Regulatory mechanism

  • MtrB: termination/antitermination, PubMed, in MtrB regulon

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed
  • the RNA switch RNA is degraded by RNase Y PubMed

• Genes: trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE
  Description: PubMed

  Regulatory mechanism

  • MtrB: termination/antitermination, PubMed, in MtrB regulon

  Regulation

  • not expressed if tryptophan is available (MtrB) PubMed
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• BKE22680 (trpE::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGCTCTCACTCCTTATG, downstream forward: _UP4_GCTGATGAACAGATTTCTAC
• BKK22680 (trpE::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGCTCTCACTCCTTATG, downstream forward: _UP4_GCTGATGAACAGATTTCTAC

References

Reviews

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The trpE RNA switch

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Other original publications

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