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rarA [2019-04-17 09:35:08]
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rarA [2019-04-17 09:35:08]

DNA-dependent ATPase, recombination factor, involved in processing recombination intermediates at replication forks
locus
BSU27530
pI
6.80
mw
46.35 kDa
protein length
421 aa Sequence Blast
gene length
1266 bp Sequence Blast
function
DNA recombination
product
DNA-dependent ATPase, recombination factor
essential
no
synonyms
yrvN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    2,812,336 2,813,601

    The protein

    Catalyzed reaction/ biological activity

  • inhibits [protein|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|PriA]-dependent initiation of [category|SW 3.1.1|DNA replication] [pubmed|29947798]

    • Protein family

  • [SW|AAA ATPase family] (according to UniProt)

    • Effectors of protein activity

  • ATPase is stimulated by ssDNAdsDNA junctions or dsDNA ends [pubmed|29947798]
  • ssDNA-dependent ATPase is stimulated by [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] bound to ssDNA [pubmed|29947798]

    • Structure

  • [PDB|3PVS] (from E. coli, 39% identity) [pubmed|21297161]

    • additional information

  • for the corresponding ''E. coli'' protein, RarA, see [http://ecoliwiki.net/colipedia/index.php/rarA RarA]

    • Expression and Regulation

    Operons

    genes
    [gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA]
    description
    [pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-A827 (yrvN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/827 NBRP B. subtilis, Japan]
  • BKE27530 ([gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATCATCTCTCCTTAC, downstream forward: _UP4_TAAAACAAAAAAACAGCCTT
  • BKK27530 ([gene|48BCA38E609E443406E03A32D2BB8DA2E9915A71|rarA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTATCATCTCTCCTTAC, downstream forward: _UP4_TAAAACAAAAAAACAGCCTT

    • References

  • 21170359,21297161,29947798