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rarA [2019-07-12 16:40:35]
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rarA [2019-07-12 16:40:35]

DNA-dependent ATPase, recombination factor, involved in processing recombination intermediates at replication forks
Locus
BSU_27530
Isoelectric point
6.80
Molecular weight
46.35 kDa
Protein length
421 aa Sequence Blast
Gene length
1266 bp Sequence Blast
Function
DNA recombination
Product
DNA-dependent ATPase, recombination factor
Essential
no
Synonyms
yrvN

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
2,812,336 2,813,601

The protein

Catalyzed reaction/ biological activity

  • inhibits PriA-dependent initiation of DNA replication PubMed
  • Protein family

  • AAA ATPase family (according to UniProt)
  • Effectors of protein activity

  • ATPase is stimulated by ssDNAdsDNA junctions or dsDNA ends PubMed
  • ssDNA-dependent ATPase is stimulated by SsbA bound to ssDNA PubMed
  • Structure

  • 3PVS (from E. coli, 39% identity) PubMed
  • Additional information

  • for the corresponding E. coli protein, RarA, see RarA
  • Expression and Regulation

    Operons

    Biological materials

    Mutant

  • MGNA-A827 (yrvN::erm), available at the NBRP B. subtilis, Japan
  • BKE27530 (rarA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATCATCTCTCCTTAC, downstream forward: _UP4_TAAAACAAAAAAACAGCCTT
  • BKK27530 (rarA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTATCATCTCTCCTTAC, downstream forward: _UP4_TAAAACAAAAAAACAGCCTT
  • References

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