vpr

minor extracellular serine protease

locus: BSU_38090

pI: 5.77

mw: 85.42 kDa

protein length: 806 aa

gene length: 2421 bp

function: protein degradation

product: minor extracellular serine protease

essential: no

synonyms: ipa-45r

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.5|Utilization of proteins]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.2|Extracellular feeding proteases]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 3,907,844 3,910,264

The protein

Protein family

• [SW|peptidase S8 family] (according to UniProt)

[SW|Domains]

• Inhibitor I9 domain (aa 57-142) (according to UniProt)
• Peptidase S8 domain (aa 158-579) (according to UniProt)

[SW|Localization]

• extracellular (signal peptide) [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|481AF959FDED9055F33524483101DA9AF2013151|vpr]
  description: [Pubmed|9353933]

  sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|25666135], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|25666135], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|16291680], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]
  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]: repression, [Pubmed|26020636], in [regulon|6740108089F13116F200C15F35C2E7561E990FEB|DnaA regulon]

  regulation

  • expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|16291680]

Biological materials

Mutant

• KO7 (''nprE aprE epr mpr nprB vpr bpr''), available as BGSC 1A1133
• BKE38090 ([gene|481AF959FDED9055F33524483101DA9AF2013151|vpr]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTTTCCCCCTTTGT, downstream forward: _UP4_TAAGAAAAAGCCCTGCCGAT
• BKK38090 ([gene|481AF959FDED9055F33524483101DA9AF2013151|vpr]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTGTTTCCCCCTTTGT, downstream forward: _UP4_TAAGAAAAAGCCCTGCCGAT

References

Reviews

• 20735481

Original publications

• 24115457,16291680,16267290,18957862,25666135,26020636,1938892