tenA [2019-07-31 09:43:26]

thiaminase II

Locus: BSU_11650

Isoelectric point: 4.99

Molecular weight: 27.27 kDa

Protein length: 236 aa

Gene length: 711 bp

Function: thiamine salvage

Product: thiaminase II

Essential: no

E.C.: 3.5.99.2

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of thiamine

Gene

Coordinates: 1,242,449 1,243,159

The protein

Catalyzed reaction/ biological activity

• Thiamine H₂O = 4-amino-5-hydroxymethyl-2-methylpyrimidine 5-(2-hydroxyethyl)-4-methylthiazole (according to Swiss-Prot)

Protein family

• TenA family (single member, according to UniProt)

Structure

• 1YAF, 2QCX (complex with formyl aminommethyl pyrimidine)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: tenA-tenI-thiO-thiS-thiG-thiF-thiD
  Description: PubMed

  Regulatory mechanism

  • Thi-box: RNA switch, via RNA switch, in Thi-box regulon

  Regulation

  • repressed by thiamine (Thi-box) PubMed
  • the Thi-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• BKE11650 (tenA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAAATCATTCCCCCTCTG, downstream forward: _UP4_AATGTGGAGCTTCACGCCAT
• BKK11650 (tenA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACAAATCATTCCCCCTCTG, downstream forward: _UP4_AATGTGGAGCTTCACGCCAT

References

Reviews

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Original publications

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