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fadN [2019-08-09 09:57:46]
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fadN [2019-08-09 09:57:46]

3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
locus
BSU_32840
pI
6.53
mw
89.96 kDa
protein length
815 aa Sequence Blast
gene length
2448 bp Sequence Blast
function
fatty acid degradation
product
3-hydroxyacyl-CoA dehydrogenase (acetoacetyl-CoA)
essential
no
ec
1.1.1.35
synonyms
yusL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

    • Gene

    Coordinates
    3,370,025 3,372,472

    The protein

    Catalyzed reaction/ biological activity

  • (S)-3-hydroxyacyl-CoA NAD = 3-oxoacyl-CoA NADH (according to Swiss-Prot)

    • Protein family

  • 3-hydroxyacyl-CoA dehydrogenase family (with [protein|FB95ABDC8557F26525E8B211595C130A7203ABC0|MmgB], according to UniProt)

    • [SW|Cofactors]

  • FMN or FAD [Pubmed|21635694]

    • Expression and Regulation

    Operons

    genes
    [gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]-[gene|07498A0A3CB598A6E388540124A4F56E781C86FA|fadA]-[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]
    description
    [Pubmed|17189250]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17189250], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR]: activation, [Pubmed|12817086], in [regulon|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR regulon]
  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|21398533], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • ''[protein|4787161A022E938A6D58A505D92369F889C7DE04|FadN]'': induced by long-chain fatty acids ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]) [Pubmed|17189250]
  • expression of the operon is induced during [SW|biofilm formation] [pubmed|31113899]
    • view in new tab

    genes
    [gene|F8C51A86268DC6B2152B139D2D0B25CC3D69A5D5|fadM]-[gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]-[gene|07498A0A3CB598A6E388540124A4F56E781C86FA|fadA]-[gene|B5325CBF1408E2CA227EB462092E209F4C87E797|fadE]
    description
    [Pubmed|17189250]

    regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]
  • [protein|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR]: activation, [Pubmed|12817086], in [regulon|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR regulon]

    • regulation

  • ''[protein|search|fadM]'': induced by long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A604 (yusL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/604 NBRP B. subtilis, Japan]
  • BKE32840 ([gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGATATCCCCCTTGAA, downstream forward: _UP4_CCTTTACGGAATTAGGAGGG
  • BKK32840 ([gene|4787161A022E938A6D58A505D92369F889C7DE04|fadN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32840 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAGATATCCCCCTTGAA, downstream forward: _UP4_CCTTTACGGAATTAGGAGGG

    • References

  • 12817086,12850135,17189250,17919287,23033921,21398533,31113899