tilS [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

trigger enzyme, tRNAIle-lysidine synthetase (modifies C34 at the wobble position of tRNA2Ile to lysidine) and part of a transcription activator

locus: BSU00670

pI: 8.00

mw: 53.00 kDa

function: tRNA modification, control of ftsH expression

product: tRNAIle-lysidine synthetase

essential: yes

ec: 6.3.4.19

synonyms: yacA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|translation], [SW|transcription factors and their control], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|essential genes]

This gene is a member of the following [SW|regulons]

• [SW|SigM regulon]

Gene

Coordinates on the chromosome (coding sequence): 74,929 -> 76,347

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• modifies C34 at the wobble position of tRNA2Ile to lysidine

Protein family

• tRNA(Ile)-lysidine synthase family (according to Swiss-Prot)

Structure

• [PDB|3A2K] (from ''Geobacillus kaustophilus'', complex with tRNA) [Pubmed|19847269]

[SW|Localization]

• Cytoplasm (Homogeneous) [Pubmed|16479537]

[SW|Interactions]

• [protein|search|TilS]-[protein|search|HprT], the complex activates transcription of ''[protein|search|ftsH]'' [Pubmed|24001521]

Expression and Regulation

Operon

• ''[protein|search|tilS]-[protein|search|hprT]-[protein|search|ftsH]'' [Pubmed|18179421]

[SW|Sigma factor]

• [protein|search|SigM] [Pubmed|18179421]

References

Reviews

• 15124629,19944692

Original publications

• 14527414,16479537,17005971,19233850,19847269,21435031,18179421,24001521,24194599