fpbP

petrobactin (3.4-catecholate siderophore) ABC transporter (ATP-binding protein)

Locus: BSU_03820

Isoelectric point: 5.09

Molecular weight: 28.33 kDa

Protein length: 252 aa

Gene length: 759 bp

Function: acquisition of iron

Product: petrobactin ABC transporter (ATP-binding protein)

Essential: no

Synonyms: yclP

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Importers → Uptake of iron/ siderophores
• Cellular processes → Homeostasis → Acquisition of iron → ABC transporters for the uptake of iron/ siderophores
• Metabolism → Additional metabolic pathways → Iron metabolism → ABC transporters for the uptake of iron/ siderophores
• Groups of genes → Membrane proteins

Gene

Coordinates: 434,256 435,014

The protein

Catalyzed reaction/ biological activity

• uptake of the siderophore petrobactin PubMed
• Fe(III)-siderophore + ATP + H₂O --> Fe(III)-siderophore + ADP + H⁺ + phosphate (according to UniProt)

Protein family

• ABC transporter superfamily (according to UniProt)

Paralogous protein(s)

• YusV, FhuC, FecF

Domains

• ABC transporter domain (aa 2-236) (according to UniProt)

Structure

• 4YMS (amino acid transporter from Caldanaerobacter subterraneus, 31% identity) PubMed

Localization

• associated to the membrane (via FpbN-FpbO) PubMed

Expression and Regulation

Operons

• Genes: fpbN-fpbO-fpbP-fpbQ
  Description: PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • immediately induced upon iron starvation (first wave to allow iron uptake) (Fur) PubMed

Biological materials

Mutant

• MGNA-C011 (yclP::erm), available at the NBRP B. subtilis, Japan
• BKE03820 (fpbP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGTTTGCTTACATTTCTGA, downstream forward: _UP4_TAATATATAGAAGAGGTGAG
• BKK03820 (fpbP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TTGTTTGCTTACATTTCTGA, downstream forward: _UP4_TAATATATAGAAGAGGTGAG

References

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