primary magnesium transporter
function
magnesium uptake
product
primary magnesium transporter
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter][category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux][category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][SW|Categories] containing this gene/protein
[SW|transporters/ other], [SW|metal ion homeostasis (K, Na, Ca, Mg)], [SW|membrane proteins]This gene is a member of the following [SW|regulons]
[SW|M-box]Gene
Coordinates on the chromosome (coding sequence)
1,396,013 -> 1,397,368
Phenotypes of a mutant
a ''[protein|search|mgtE]'' mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg2, moreover the mutant grows well on minimal medim in the presence of citrate [Pubmed|24415722]a ''[protein|search|mgtE] [protein|search|yfjQ]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2, moreover the mutant grows well on minimal medim in the presence of citrate [Pubmed|24415722] The protein
Protein family
SLC41A transporter family (according to Swiss-Prot)[SW|Domains]
2 [SW|CBS domain]s (140-203, 204-260)Structure
[PDB|2YVX] (from ''Thermus thermophilus'', 34% identity) [Pubmed|17700703][PDB|2QBZ] (the mgtE riboswitch)[SW|Localization]
cell membrane (according to Swiss-Prot)Expression and Regulation
Regulation
induced at low magnesium concentration ([protein|search|M-box]) [Pubmed|17803910]Regulatory mechanism
[protein|search|M-box]: Mg(2)-responsive [SW|riboswitch] [Pubmed|17803910] Biological materials
Mutant
MGNA-B313 (ykoK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1312 NBRP B. subtilis, Japan]Expression vector
pGP2927 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s labReferences
17803910,15096624,21315082,22970223,24415722,25182490,17700703,25367295 
