mgtE [2019-08-28 09:19:20]

primary magnesium transporter

locus: BSU_13300

pI: 4.61

mw: 50.68 kDa

protein length: 451 aa

gene length: 1356 bp

function: magnesium uptake

product: primary magnesium transporter

essential: yes

synonyms: ykoK

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
• [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,396,013 1,397,368

Phenotypes of a mutant

• essential [Pubmed|28189581]
• a ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]'' mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg²⁺, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|CitM]) [Pubmed|24415722]
• a ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE] [gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg²⁺, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|CitM]) [Pubmed|24415722]

The protein

Protein family

• SLC41A transporter family (single member, according to UniProt)

[SW|Domains]

• 2 [SW|CBS domain]s (140-203, 204-260)

Effectors of protein activity

• binds c-di-AMP [pubmed|31061098]

Structure

• [PDB|2YVX] (from ''Thermus thermophilus'', 34% identity) [Pubmed|17700703]
• [PDB|2QBZ] (the mgtE riboswitch)

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|M-box|M-box]: termination/antitermination, Mg-responsive [protein|C245476CE35028167510F67153F392D91447553F|MgtE riboswitch], in [regulon|M-box|M-box]

  regulation

  • induced at low magnesium concentration ([regulon|M-box|M-box]) [Pubmed|17803910]

additional information

• expression is increased as a result of loop duplication in [protein|F3DE42C41EF57DC5DFFF0D28129F2CE5DB781D99|L22] [pubmed|30853217]

Biological materials

Mutant

• MGNA-B313 (mgtE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1312 NBRP B. subtilis, Japan]
• GP2854 (''Δ''''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]''::''ermC''), available in [SW|Jörg Stülke]'s lab
• GP2762 (''Δ''''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]''::''spec''), available in [SW|Jörg Stülke]'s lab
• GP2763 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''kan'' ''Δ''''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]''::''spec''), available in [SW|Jörg Stülke]'s lab

Expression vectors

• pGP2927 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

lacZ fusion

• GP2869 (based on [SW|pAC5]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP2768 ''mgtE-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 24079267

Original publications

• 17803910,15096624,21315082,22970223,24415722,25182490,17700703,25367295,28189581,19798051,17671366,28747715,18832160,29967120,30853217,31061098