yvqK

ATP:cob(I)alamin adenosyltransferase

Locus: BSU_33150

Isoelectric point: 5.21

Molecular weight: 21.34 kDa

Protein length: 193 aa

Gene length: 582 bp

Function: unknown

Product: ATP:cob(I)alamin adenosyltransferase

Essential: no

E.C.: 2.5.1.17

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of cobalamine (B12))
• Groups of genes → Poorly characterized/ putative enzymes

Gene

Coordinates: 3,400,537 3,401,118

The protein

Catalyzed reaction/ biological activity

• 2 ATP + 2 cob(II)yrinate a,c diamide + reduced [electron-transfer flavoprotein] --> 2 adenosylcob(III)yrinate a,c-diamide + 3 H⁺ + oxidized [electron-transfer flavoprotein] + 2 triphosphate (according to UniProt)
• 2 ATP + 2 cob(II)alamin + reduced [electron-transfer flavoprotein] --> 2 adenosylcob(III)alamin + 3 H⁺ + oxidized [electron-transfer flavoprotein] + 2 triphosphate (according to UniProt)

Protein family

• Cob(I)alamin adenosyltransferase family (single member, according to UniProt)

Structure

• 1RTY

Expression and Regulation

Operons

• Genes: yvrC-yvrB-yvrA-yvqK

  Regulatory mechanism

  • B12 riboswitch: termination, upon binding of B12 PubMed, in B12 riboswitch

  Regulation

  • expression is decreased in the presence of cobalamin (vitamin B12) (B12 riboswitch) PubMed

Biological materials

Mutant

• MGNA-B042 (yvqK::erm), available at the NBRP B. subtilis, Japan
• BKE33150 (yvqK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACCCTGAATCTCCTTTA, downstream forward: _UP4_TAAAAAGTGAAAAGGAAAGC
• BKK33150 (yvqK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACCCTGAATCTCCTTTA, downstream forward: _UP4_TAAAAAGTGAAAAGGAAAGC

References

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