psdA

ABC transporter (ATP-binding protein) for the export of lipid II-binding lantibiotics, such as nisin and gallidermin

Locus: BSU_34700

Isoelectric point: 6.34

Molecular weight: 28.89 kDa

Protein length: 259 aa

Gene length: 780 bp

Function: export of toxic peptides

Product: ABC transporter (ATP-binding protein)

Essential: no

Synonyms: yvcR

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ABC transporters → Exporters → Export of peptides
• Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,565,496 3,566,275

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Paralogous protein(s)

• YxdL, FtsE, BceA, YknY, YvrO

Domains

• ABC transporter domain (aa 4-248) (according to UniProt)

Structure

• 1L2T (MJ0796 from Methanocaldococcus jannaschii , 44% identity) PubMed

Localization

• membrane associated (via PsdB) PubMed

Expression and Regulation

Operons

• Genes: psdR-psdS-psdA-psdB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PsdR: activation, PubMed, in PsdR regulon

  Regulation

  • psdA: induced by nisin, gallidermin (PsdR) PubMed

• Genes: psdA-psdB
  Description: PubMed

  Regulation

  • psdA: induced by nisin, gallidermin (PsdR) PubMed

Biological materials

Mutant

• MGNA-B635 (yvcR::erm), available at the NBRP B. subtilis, Japan
• BKE34700 (psdA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCTGCTCCTCCTTTT, downstream forward: _UP4_TTGAGTGTGTTGGGAGGCGA
• BKK34700 (psdA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTCTGCTCCTCCTTTT, downstream forward: _UP4_TTGAGTGTGTTGGGAGGCGA

References

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