comFA

membrane-associated DNA-dependent ATPase, may provide energy for DNA transport

Locus: BSU_35470

Isoelectric point: 9.82

Molecular weight: 52.40 kDa

Protein length: 463 aa

Gene length: 1392 bp

Function: genetic competence

Product: ATP-binding protein

Essential: no

Synonyms

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Genetics → Genetic competence
• Lifestyles → Exponential and early post-exponential lifestyles → Genetic competence
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 3,642,167 3,643,558

Phenotypes of a mutant

• knockout has 1000-fold reduction in transformability compared to the wild-type PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• helicase family (according to UniProt)

Domains

• Helicase ATP-binding domain (aa 133-285) (according to UniProt)
• Helicase C-terminal domain (aa 317-463) (according to UniProt)

Modification

• phosphorylated on Arg-20, Arg-186, and Arg-446 PubMed

Cofactors

• Zn²⁺ PubMed

Localization

• cell membrane PubMed
• localizes to cell poles in competent cells PubMed
• localizes to cell poles PubMed

Expression and Regulation

Operons

• Genes: comFA-comFB-comFC-yvyF-flgM-yvyG-flgK-flgL-yviE-fliW-csrA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • ScoC: repression, PubMed, in ScoC regulon

Biological materials

Mutant

• BKE35470 (comFA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATAGCACGCCTCCTTTC, downstream forward: _UP4_TAGCCATTATTTGAAAACGT
• BKK35470 (comFA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACATAGCACGCCTCCTTTC, downstream forward: _UP4_TAGCCATTATTTGAAAACGT

References

Reviews

Loading

Original publications

Loading