mdtP [2018-10-04 20:32:08]

multidrug-efflux transporter

locus: BSU32880

pI: 9.84

mw: 57.78 kDa

protein length: 541 aa

gene length: 1623 bp

function: multidrug resistance

product: multidrug-efflux transporter

essential: no

synonyms: yusP

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.3|Carbohydrate transporter]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,374,956 → 3,376,581

The protein

Protein family

• EmrB family (according to Swiss-Prot)

[SW|Localization]

• cell membrane (according to Swiss-Prot), membrane associated [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|795D70E8A4D750140D6B1AA4BFEBD25C2A389C85|mdtR]-[gene|45C61C1584A851676F15A2249F3F1092863C5AA5|mdtP]
  description: [Pubmed|19286808]

  regulatory mechanism

  • [protein|795D70E8A4D750140D6B1AA4BFEBD25C2A389C85|MdtR]: repression, [Pubmed|19286808], in [regulon|795D70E8A4D750140D6B1AA4BFEBD25C2A389C85|MdtR regulon]

Biological materials

Mutant

• MGNA-A601 (yusP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/601 NBRP B. subtilis, Japan]
• BP70 (spc) available in [SW|Fabian Commichau]'s lab
• BKE32880 (Δ[gene|45C61C1584A851676F15A2249F3F1092863C5AA5|mdtP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGTTTGCCTTCTTTACTCA, downstream forward: _UP4_TAATAGAGCACCCCGCGGGT
• BKK32880 (Δ[gene|45C61C1584A851676F15A2249F3F1092863C5AA5|mdtP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTGTTTGCCTTCTTTACTCA, downstream forward: _UP4_TAATAGAGCACCCCGCGGGT

References

• 18763711,19286808