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pdhB [2017-8-11 16:0:3]
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pdhB [2017-8-11 16:0:3]

pyruvate dehydrogenase (E1 beta subunit)
Locus
BSU14590
Isoelectric point
4.55
Molecular weight
35.32 kDa
Protein length
325 aa Sequence Blast
Gene length
975 bp Sequence Blast
Function
links glycolysis and TCA cycle
Product
pyruvate dehydrogenase (E1 beta subunit)
Essential
no
E.C.
1.2.4.1
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Carbon metabolism → Carbon core metabolism → TCA cycle
  • Information processing → Genetics → Newly identified competence genes
  • Groups of genes → Membrane proteins
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

    • Gene

    Coordinates
    1,529,445 → 1,530,422

    Phenotypes of a mutant

  • defects in sporulation and unable to grow on glucose as single carbon source PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Pyruvate [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine CO2 (according to Swiss-Prot)

    • Paralogous protein(s)

  • AcoB, BkdAB

    • Kinetic information

  • Michaelis-Menten PubMed

    • Modification

  • phosphorylation on (Ser-302 OR Ser-306) PubMed

    • Effectors of protein activity

  • Inhibited thiamine 2-thiothiazolone diphosphate and NADH PubMed
  • Low sensibility to NADPH

    • Structure

  • 1W88 (E1 in complex with subunit binding domain of E2, Geobacillus stearothermophilus)

    • Localization

  • membrane associated PubMed

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    pdhA-pdhB-pdhC-pdhD
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • stringent response: negative regulation, due to presence of guanine at 1 position of the transcript PubMed, in stringent response

    • Regulation

  • pdhA: expression activated by glucose (1.9-fold) PubMed
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    Biological materials

    Mutant

  • GP459 (spc), available in Stülke lab

    • LacZ fusion

  • pGP722 (in pAC5), available in Stülke lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab

    • Labs working on this gene/protein

  • Arthur Aronson, Purdue University, West Lafayette, USA homepage

    • References

    Reviews

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    Original publications

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