pdhB

pyruvate dehydrogenase (E1 beta subunit)

Locus: BSU_14590

Isoelectric point: 4.55

Molecular weight: 35.32 kDa

Protein length: 325 aa

Gene length: 978 bp

Function: links glycolysis and TCA cycle

Product: pyruvate dehydrogenase (E1 beta subunit)

Essential: no

E.C.: 1.2.4.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Carbon core metabolism → TCA cycle
• Information processing → Genetics → Newly identified competence genes
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

Gene

Coordinates: 1,529,445 1,530,422

Phenotypes of a mutant

• defects in sporulation and unable to grow on glucose as single carbon source PubMed
• poor growth PubMed
• non-transformable PubMed

The protein

Catalyzed reaction/ biological activity

• [dihydrolipoyllysine-residue acetyltransferase]-(R)-N⁶-lipoyl-L-lysine + H⁺ + pyruvate --> [dihydrolipoyllysine-residue acetyltransferase]-(R)-N⁶-(S⁸-acetyldihydrolipoyl)-L-lysine + CO₂ (according to UniProt)

Paralogous protein(s)

• AcoB, BkdAB

Kinetic information

• Michaelis-Menten PubMed

Modification

• phosphorylation on (Ser-302 OR Ser-306) PubMed

Effectors of protein activity

• Inhibited thiamine 2-thiothiazolone diphosphate and NADH PubMed
• Low sensibility to NADPH

Structure

• 1W88 (E1 in complex with subunit binding domain of E2, Geobacillus stearothermophilus)

Localization

• membrane associated PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: pdhA-pdhB-pdhC-pdhD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • stringent response: negative regulation, due to presence of guanine at 1 position of the transcript PubMed, in stringent response

  Regulation

  • pdhA: expression activated by glucose (1.9-fold) PubMed

Biological materials

Mutant

• GP459 (spc), available in Jörg Stülke's lab
• BKE14590 (pdhB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCATTGTCATTTGCGCCA, downstream forward: _UP4_TAATCAAACTGCATAATCGA
• BKK14590 (pdhB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCATTGTCATTTGCGCCA, downstream forward: _UP4_TAATCAAACTGCATAATCGA

LacZ fusion

• pGP722 (in pAC5), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Labs working on this gene/protein

• Arthur Aronson, Purdue University, West Lafayette, USA homepage

References

Reviews

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Original publications

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