yciB

putative L,D-transpeptidase

Locus: BSU_03350

Isoelectric point: 6.18

Molecular weight: 21.94 kDa

Protein length: 194 aa

Gene length: 585 bp

Function: unknown

Product: putative L,D-transpeptidase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall synthesis → Biosynthesis of peptidoglycan
• Metabolism → Additional metabolic pathways → Biosynthesis of cell wall components → Biosynthesis of peptidoglycan
• Groups of genes → Membrane proteins

Gene

Coordinates: 365,170 365,754

The protein

Protein family

• YkuD family (with Ldt and YqjB, according to UniProt)

Structure

• 4LZH (from Klebsiella pneumoniae, 27% identity)

Localization

• lipoprotein, cell membrane at the cell poles PubMed

Expression and Regulation

Operons

• Genes: folEB-yciB-yczL-zagA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Zur: repression, in the presence of zinc PubMed, in Zur regulon

  Regulation

  • folE2: induced by zinc starvation (Zur) PubMed

Biological materials

Mutant

• MGNA-B998 (yciB::erm), available at the NBRP B. subtilis, Japan
• BKE03350 (yciB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGCAATAATGAATAATGAGA, downstream forward: _UP4_TGAATTGAAAACCGTTGACA
• BKK03350 (yciB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_TGCAATAATGAATAATGAGA, downstream forward: _UP4_TGAATTGAAAACCGTTGACA

References

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