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gltA [2019-02-05 18:26:00]
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gltA [2019-02-05 18:26:00]

large subunit of glutamate synthase
locus
BSU18450
pI
5.47
mw
168.61 kDa
protein length
1520 aa Sequence Blast
gene length
4563 bp Sequence Blast
function
glutamate biosynthesis
product
glutamate synthase (large subunit)
essential
no
ec
1.4.1.13
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
  • [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • Gene

    Coordinates
    2,010,070 2,014,632

    Phenotypes of a mutant

  • auxotrophic for glutamate

    • The protein

    Catalyzed reaction/ biological activity

  • 2 L-glutamate + NADP+ --> L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot)

    • Protein family

  • glutamate synthase family (according to Swiss-Prot) glutamate synthase family

    • Paralogous protein(s)

  • [protein|D1AF9DA2594D2E2A94DC207A870C8495954A7A17|YerD]

    • [SW|Domains]

  • Glutamine amidotransferase type-2 domain (22-415)
  • Nucleotide binding domain (1060-1112)

    • Modification

  • phosphorylated on Arg-904 AND/OR Arg-914 [Pubmed|22517742]

    • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]
  • FAD, FMN

    • Structure

  • [PDB|2VDC] (the [protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|GltA]-[protein|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|GltB] complex of ''Azospirillum brasiliense'') [Pubmed|18199747]

    • [SW|Localization]

  • membrane associated [pubmed|18763711], cytoplasm (according to UniProt)

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • [SW|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp] due to the presence of several consecutive proline residues [Pubmed|23239624,23239623]

    • Expression and Regulation

    Operons

    genes
    [gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]
    description
    [Pubmed|12823818]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2548995], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|11029411], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|87BCAE725B02860156D50E1783F6DB68510C811E|GltC]: activation, [Pubmed|2548995], in [regulon|87BCAE725B02860156D50E1783F6DB68510C811E|GltC regulon]
  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: indirect effect, in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • expressed in the presence of ammonium [Pubmed|11029411]
    • view in new tab

    Other regulations

  • [protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|FsrA]: translation inhibition, [Pubmed|22389480]

    • Biological materials

    Mutant

  • GP807 (del ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]''::''tet'') , available in [SW|Jrg Stlke]'s lab
  • GP222 (''gltA'' under the control of p-xyl), available in [SW|Jrg Stlke]'s lab
  • 1A808 ( ''gltA''::''cat''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A808&Search=1A808 BGSC]
  • 1A809 ( ''gltA''::''kan''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A809&Search=1A809 BGSC]
  • BKE18450 ([gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGA
  • BKK18450 ([gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGA

    • lacZ fusion

  • pGP526 (in [protein|search|pAC7]), available in [SW|Jrg Stlke]'s lab [pubmed|14523131]
  • pGP919 (in [protein|search|pAC5]), available in [SW|Jrg Stlke]'s lab [pubmed|17183217]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jrg Stlke]'s lab

    • labs

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • [SW|Jrg Stlke], University of Gttingen, Germany
  • [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • [SW|Fabian Commichau] University of Gttingen, Germany
  • [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]

    • References

    Reviews

  • 16143852,12859215,22625175,12859215

    • Original publications

  • 12823818,18199747,11029411,12850135,7559360,15150225,2548995,17183217,17608797,17134717,14523131,12823818,18326565,17981983,18763711,20933603,17012385,22389480,22517742,25755103,28294562,28386026,29242163