large subunit of glutamate synthase
function
glutamate biosynthesis
product
glutamate synthase (large subunit)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation][category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]Gene
Coordinates
2,010,070 2,014,632
Phenotypes of a mutant
auxotrophic for glutamateThe protein
Catalyzed reaction/ biological activity
2 L-glutamate + NADP+ --> L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot)Protein family
glutamate synthase family (according to Swiss-Prot) glutamate synthase familyParalogous protein(s)
[protein|D1AF9DA2594D2E2A94DC207A870C8495954A7A17|YerD] [SW|Domains]
Glutamine amidotransferase type-2 domain (22-415)Nucleotide binding domain (1060-1112)Modification
phosphorylated on Arg-904 AND/OR Arg-914 [Pubmed|22517742][SW|Cofactors]
Fe-S cluster [pubmed|29292548]FAD, FMNStructure
[PDB|2VDC] (the [protein|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|GltA]-[protein|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|GltB] complex of ''Azospirillum brasiliense'') [Pubmed|18199747][SW|Localization]
membrane associated [pubmed|18763711], cytoplasm (according to UniProt)Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983][SW|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp] due to the presence of several consecutive proline residues [Pubmed|23239624,23239623]Expression and Regulation
Operons
genes
[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]
description
[Pubmed|12823818]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2548995], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: repression, [Pubmed|11029411], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon][protein|87BCAE725B02860156D50E1783F6DB68510C811E|GltC]: activation, [Pubmed|2548995], in [regulon|87BCAE725B02860156D50E1783F6DB68510C811E|GltC regulon][protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: indirect effect, in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]regulation
expressed in the presence of ammonium [Pubmed|11029411]view in new tabOther regulations
[protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|FsrA]: translation inhibition, [Pubmed|22389480]Biological materials
Mutant
BP123 (Δ[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]::''ermC'') , available in [SW|Fabian Commichau]'s labGP807 (Δ[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]::''tet'') , available in [SW|Jörg Stülke]'s labGP222 (''gltA'' under the control of p-xyl), available in [SW|Jörg Stülke]'s lab1A808 ( ''gltA''::''cat''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A808&Search=1A808 BGSC]1A809 ( ''gltA''::''kan''), [Pubmed|15109830], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A809&Search=1A809 BGSC]BKE18450 ([gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGABKK18450 ([gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGAlacZ fusion
pGP526 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab [pubmed|14523131]pGP919 (in [protein|search|pAC5]), available in [SW|Jörg Stülke]'s lab [pubmed|17183217]two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lablabs
[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage][SW|Jörg Stülke], University of Gttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage][SW|Fabian Commichau] University of Gttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]References
Reviews
16143852,12859215,22625175,12859215 Original publications
12823818,18199747,11029411,12850135,7559360,15150225,2548995,17183217,17608797,17134717,14523131,12823818,18326565,17981983,18763711,20933603,17012385,22389480,22517742,25755103,28294562,28386026,29242163