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gltA [2019-02-19 17:28:51]
large subunit of glutamate synthase
Molecular weight
168.61 kDa
Function
glutamate biosynthesis
Product
glutamate synthase (large subunit)
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
2,010,070 2,014,632
Phenotypes of a mutant
auxotrophic for glutamateThe protein
Catalyzed reaction/ biological activity
2 L-glutamate + NADP+ --> L-glutamine + 2-oxoglutarate + NADPH (according to Swiss-Prot)Protein family
glutamate synthase family (according to Swiss-Prot) glutamate synthase familyParalogous protein(s)
Domains
Glutamine amidotransferase type-2 domain (22-415)Nucleotide binding domain (1060-1112)Modification
phosphorylated on Arg-904 AND/OR Arg-914 PubMed Cofactors
Structure
Localization
membrane associated PubMed, cytoplasm (according to UniProt) Additional information
subject to Clp-dependent proteolysis upon glucose starvation PubMedtranslation is likely to require Efp due to the presence of several consecutive proline residues PubMed Expression and Regulation
Biological materials
Mutant
BP123 (ΔgltA-gltB::ermC) , available in Fabian Commichau's labGP807 (ΔgltA-gltB::tet) , available in Jörg Stülke's labGP222 (gltA under the control of p-xyl), available in Jörg Stülke's lab1A808 ( gltA::cat), PubMed, available at BGSC1A809 ( gltA::kan), PubMed, available at BGSCBKE18450 (gltA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGABKK18450 (gltA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATTCCTCTCCCCCGAT, downstream forward: _UP4_GTACAGTAAGGAAGGGGAGA LacZ fusion
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab Labs working on this gene/protein
References
Reviews
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Original publications
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