gltA [2017-06-19 17:41:58]

large subunit of glutamate synthase

Locus: BSU18450

Isoelectric point: 5.47

Molecular weight: 168.61 kDa

Protein length: 1520 aa

Gene length: 4560 bp

Function: glutamate biosynthesis

Product: glutamate synthase (large subunit)

Essential: no

E.C.: 1.4.1.13

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Amino acid/ nitrogen metabolism → Biosynthesis/ acquisition of amino acids → Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation
• Groups of genes → Efp-dependent proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Gene

Coordinates: 2,010,070 → 2,014,632

Phenotypes of a mutant

• auxotrophic for glutamate

The protein

Catalyzed reaction/ biological activity

• 2 L-glutamate NADP = L-glutamine 2-oxoglutarate NADPH (according to Swiss-Prot) 2 L-glutamate NADP( ) <=> L-glutamine 2-oxoglutarate NADPH

Protein family

• glutamate synthase family (according to Swiss-Prot) glutamate synthase family

Paralogous protein(s)

• YerD

Domains

• Glutamine amidotransferase type-2 domain (22-415)
• Nucleotide binding domain (1060-1112)

Modification

• phosphorylated on Arg-904 AND/OR Arg-914 PubMed

Cofactors

• [3 Fe- 4 S] cluster, FAD, FMN

Structure

• 2VDC (the GltA-GltB complex of Azospirillum brasiliense) PubMed

Localization

• membrane associated 18763711 PubMed, cytoplasm

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed
• translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Expression and Regulation

Operons

• Genes: gltA-gltB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • TnrA: repression, PubMed, in TnrA regulon
  • GltC: activation, PubMed, in GltC regulon
  • Fur: indirect effect, in Fur regulon

  Regulation

  • expressed in the presence of ammonium PubMed

Other regulations

• FsrA: translation inhibition, PubMed

Biological materials

Mutant

• GP807 (del gltA-gltB::tet) , available in Jörg Stülke's lab
• GP222 (gltA under the control of p-xyl), available in Jörg Stülke's lab
• 1A808 ( gltA::cat), PubMed, available at BGSC
• 1A809 ( gltA::kan), PubMed, available at BGSC

LacZ fusion

• pGP526 (in pAC7), available in Jörg Stülke's lab PubMed
• pGP919 (in pAC5), available in Jörg Stülke's lab PubMed

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Labs working on this gene/protein

• Linc Sonenshein, Tufts University, Boston, MA, USA Homepage
• Jörg Stülke, University of Göttingen, Germany
• Homepage
• Fabian Commichau University of Göttingen, Germany
• Homepage

References

Reviews

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Original publications

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