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rplA [2019-02-19 19:04:37]
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rplA [2019-02-19 19:04:37]

ribosomal protein
Locus
BSU01030
Isoelectric point
9.73
Molecular weight
24.77 kDa
Protein length
232 aa Sequence Blast
Gene length
699 bp Sequence Blast
Function
translation
Product
ribosomal protein L1 (BL1)
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → Ribosomal proteins

    • Gene

    Coordinates
    119,111 119,809

    Phenotypes of a mutant

  • slow growth, this can be suppressed by inactivation of mpfA PubMed
  • reduced sporulation frequency, this can be suppressed by inactivation of mpfA PubMed

    • The protein

    Protein family

  • ribosomal protein L1P family (according to Swiss-Prot)

    • Structure

  • 3J9W (the ribosome) PubMed

    • Expression and Regulation

    Operons

    Genes
    rplK-rplA-BSU_misc_RNA_3-rplJ-rplL
    Description
    PubMed

    Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

    • Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
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    Biological materials

    Mutant

  • BKE01030 (rplA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTAAAATTTCCTCCTT, downstream forward: _UP4_TAATATTGACATGACATCAA
  • BKK01030 (rplA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTAAAATTTCCTCCTT, downstream forward: _UP4_TAATATTGACATGACATCAA

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab

    • FLAG-tag construct

  • GP1094 (erm, based on pGP1087), available in Jrg Stlke's lab

    • References

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