rplA

ribosomal protein

locus: BSU_01030

pI: 9.73

mw: 24.77 kDa

protein length: 232 aa

gene length: 699 bp

function: translation

product: ribosomal protein L1 (BL1)

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]

Gene

Coordinates: 119,111 119,809

Phenotypes of a mutant

• slow growth, this can be suppressed by inactivation of [gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA] [pubmed|29967120]
• reduced sporulation frequency, this can be suppressed by inactivation of [gene|BE0777DE7C40825D4DB7252EA84AAB3892578529|mpfA] [pubmed|29967120]

The protein

Protein family

• universal [SW|ribosomal protein] uL1 family (single member, according to UniProt)

Structure

• [PDB|3J9W] (the [SW|ribosome]) [Pubmed|25903689]

[SW|Localization]

• [SW|ribosome] (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|F94A1C14912F35ADB4C611AA17D55268CC26160D|rplK]-[gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA]-[gene|E4D89640D0F0A31A2814D20FB5425FDB0B46A43D|BSU_misc_RNA_3]-[gene|5EF3A7953AA97D2B44C967542A53FEA7F36D7BA3|rplJ]-[gene|E72910604D3DC2A62AE8A951F55570EA2838B250|rplL]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

Biological materials

Mutant

• BKE01030 ([gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAAAATTTCCTCCTT, downstream forward: _UP4_TAATATTGACATGACATCAA
• BKK01030 ([gene|44D4CE353E1E532664A508CBE8B49AC8BB861B53|rplA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAAAATTTCCTCCTT, downstream forward: _UP4_TAATATTGACATGACATCAA

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP1094 (erm, based on [SW|pGP1087]), available in [SW|Jörg Stülke]'s lab

References

• 17981968,19653700,11463749,22848659,23175651,23002217,25903689,29967120