purC [2019-07-23 11:46:03]

phosphoribosylaminoimidazole succinocarboxamide synthase

Locus: BSU_06450

Isoelectric point: 4.88

Molecular weight: 27.31 kDa

Protein length: 241 aa

Gene length: 726 bp

Function: purine biosynthesis

Product: phosphoribosylaminoimidazole succinocarboxamide synthase

Essential: no

E.C.: 6.3.2.6

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides

Gene

Coordinates: 701,601 702,326

The protein

Catalyzed reaction/ biological activity

• ATP 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate L-aspartate = ADP phosphate (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (according to Swiss-Prot)

Protein family

• SAICAR synthetase family (according to Swiss-Prot)

Structure

• 1KUT (from Thermotoga maritima, 40% identity, 57% similarity) PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: purE-purK-purB-purC-purS-purQ-purL-purF-purM-purN-purH-purD
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • G-box: termination, (riboswitch) PubMed, in G-box
  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon

  Regulation

  • expression activated by glucose (4.4 fold) PubMed

Additional information

• the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

• BKE06450 (purC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGGAAGGCCTCCTAACCC, downstream forward: _UP4_TTCAATAGACTGGGAGGCAT
• BKK06450 (purC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGGAAGGCCTCCTAACCC, downstream forward: _UP4_TTCAATAGACTGGGAGGCAT

References

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