zwf [2017-8-11 16:0:3]

glucose 6-phosphate dehydrogenase, pentose-phosphate pathway

locus: BSU23850

pI: 5.28

mw: 55.49 kDa

protein length: 489 aa

gene length: 1467 bp

function: initiation of the pentose phosphate pathway

product: glucose 6-phosphate dehydrogenase

essential: no

ec: 1.1.1.49

synonyms: yqjJ

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.3|Pentose phosphate pathway]

Gene

Coordinates: 2,479,156 → 2,480,625

The protein

Catalyzed reaction/ biological activity

• D-glucose 6-phosphate + NADP⁺ = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)

Protein family

• glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)

[SW|Cofactors]

• Mg2+, Mn2+, Ca2+

Effectors of protein activity

• NAD+, NADP+ and NADPH affect the enzyme kinetic [Pubmed|6292660]

Structure

• [PDB|1DPG] (from ''Leuconostoc Mesenteroides'', 45% identity, 62% similarity) [Pubmed|7881907]

Additional information

• The enzyme is a dimer [Pubmed|6292660]

Expression and Regulation

Operons

• genes: [gene|43CD2581DA421CABC66E30389640CC86E6B2D800|zwf]
  description: [Pubmed|12850135]

  regulation

  • constitutive [Pubmed|12850135]

Biological materials

Mutant

• MGNA-C392 (yqjJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2390 NBRP B. subtilis, Japan]
• SM-NB3 (''zwf-spc''), available in [SW|Anne Galinier]'s and [SW|Boris Görke]'s labs

FLAG-tag construct

• GP1401 (spc, based on [SW|pGP1331]), available in the [SW|Stülke] lab

Antibody

• **

References

• 6292660,12850135,19779711,7881907,24571712