ymfF [2019-04-14 15:19:14]

unknown

locus: BSU16845

pI: 5.26

mw: 48.00 kDa

protein length: 426 aa

gene length: 1281 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 1,757,037 1,758,317

The protein

Structure

• [PDB|3D3Y] (from Enterococcus faecalis, 34% identity)

Expression and Regulation

Operons

• genes: [gene|11997FE6BA6947E7C7E3DBE20B49C9FB758262B2|ymfD]-[gene|43BADA6A6C8248270A50EB3D81253ECE6C84F03F|ymfF]-[gene|9A5EB7CA38D948007AD788297128035AB4074490|ymfH]
  description: [Pubmed|18502870]

  regulatory mechanism

  • [protein|A7326377132C670B60695EFE0A652B1E4F623698|Mta]: activation, [Pubmed|18502870], in [regulon|A7326377132C670B60695EFE0A652B1E4F623698|Mta regulon]

Biological materials

Mutant

• MGNA-B117 (ymfF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1116 NBRP B. subtilis, Japan]
• BKE16845 ([gene|43BADA6A6C8248270A50EB3D81253ECE6C84F03F|ymfF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16845 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGGAACCTCCTTCGTT, downstream forward: _UP4_TTAAAAGGGACGGAGGGTGC
• BKK16845 ([gene|43BADA6A6C8248270A50EB3D81253ECE6C84F03F|ymfF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16845 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGGAACCTCCTTCGTT, downstream forward: _UP4_TTAAAAGGGACGGAGGGTGC

References

• 18502870