yhdK

anti-[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM] protein,required for the perception of cell wall stress signals

locus: BSU_09500

pI: 8.13

mw: 10.50 kDa

protein length: 96 aa

gene length: 291 bp

function: control of [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM] activity

product: anti-[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM] protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,028,233 1,028,523

Phenotypes of a mutant

• small colonies on LB [pubmed|30715747]

The protein

[SW|Localization]

• cell membrane [Pubmed|14993308]

Expression and Regulation

Operons

• genes: [gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]-[gene|48975EC8FB5B03F136AA112DE2ED9CFD0E9C4141|yhdL]-[gene|43AD66E4AEE951130F134FD720E17DB4D2FF112E|yhdK]
  description: [Pubmed|9573210]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9573210], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|9573210,18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

  regulation

  • induced by glucose [Pubmed|27965645]

  additional information

  • expression of [protein|search|CsbB] in the absence of [protein|search|YfhO] causes constitutive activation of [protein|search|SigM] [PubMed|23632331]

Biological materials

Mutant

• MGNA-A694 (yhdK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/694 NBRP B. subtilis, Japan]
• BP97 (Δ([gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]-[gene|48975EC8FB5B03F136AA112DE2ED9CFD0E9C4141|yhdL]-[gene|43AD66E4AEE951130F134FD720E17DB4D2FF112E|yhdK])::aphA3), available in [SW|Fabian Commichau]'s lab
• BKE09500 ([gene|43AD66E4AEE951130F134FD720E17DB4D2FF112E|yhdK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACATTGTGTTCTTTAAAAA, downstream forward: _UP4_TAAAAAAGACGCCTTTTCAG
• BKK09500 ([gene|43AD66E4AEE951130F134FD720E17DB4D2FF112E|yhdK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09500 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACATTGTGTTCTTTAAAAA, downstream forward: _UP4_TAAAAAAGACGCCTTTTCAG

References

Reviews

• 27344142,29343670

Original publications

• 18179421,14993308,9573210,30715747