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mprF [2019-06-26 07:52:31]
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mprF [2019-06-26 07:52:31]

synthesis of lysylphosphatidylglycerol (L-PG), lysinylation of phosphatidylglycerol provides some resistance against positively charged antimicrobial peptides
locus
BSU08425
pI
9.00
mw
96.00 kDa
protein length
856 aa Sequence Blast
gene length
2571 bp Sequence Blast
function
lysyl-phosphatidylglycerol lipid biosynthesis
product
lysyl-phosphatidylglycerol synthase
essential
no
synonyms
yfiW,yfiX
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.2|Biosynthesis of phospholipids]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    916,778 919,348

    Phenotypes of a mutant

  • reduced conjugation of ICEBs1 [Pubmed|25069588,26833415]
  • overexpression results in increased conjugation of ICEBs1 [Pubmed|26833415]

    • The protein

    Structure

  • [PDB|4V36] (Lys-tRNA(Lys)-dependent lysyl-phosphatidylglycerol synthase domain from ''B. licheniformis'', 76% identity) [Pubmed|26261323]

    • [SW|Localization]

  • cell membrane at the septum [Pubmed|15743965]

    • Expression and Regulation

    Operons

    genes
    [gene|4385EA135BDEB54B33C9A8E39150A60C83C0CEEA|mprF]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C310 (yfiX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2308 NBRP B. subtilis, Japan]
  • GP2246 (''[gene|4385EA135BDEB54B33C9A8E39150A60C83C0CEEA|mprF]''::''ermC''), available in [SW|Jörg Stülke]'s lab
  • BKE08425 ([gene|4385EA135BDEB54B33C9A8E39150A60C83C0CEEA|mprF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08425 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGGTCTCTCCAATCATA, downstream forward: _UP4_GTCTAATAAGACGGAGTCTT
  • BKK08425 ([gene|4385EA135BDEB54B33C9A8E39150A60C83C0CEEA|mprF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08425 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGGTCTCTCCAATCATA, downstream forward: _UP4_GTCTAATAAGACGGAGTCTT

    • References

  • 19164152,18820022,19734140,18305156,12427923,15743965,25069588,26261323,26833415