mtnW [2017-8-11 16:0:3]

2,3-diketo-5-methylthiopentyl-1-phosphate enolase, Rubisco-like protein

locus: BSU13590

pI: 5.28

mw: 44.89 kDa

protein length: 414 aa

gene length: 1242 bp

function: methionine salvage

product: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase

essential: no

synonyms: ykrW

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]

Gene

Coordinates: 1,427,034 → 1,428,278

The protein

Catalyzed reaction/ biological activity

• 5-(methylthio)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate (according to Swiss-Prot)

Protein family

• Type IV subfamily (according to Swiss-Prot)

Structure

• [PDB|2ZVI] [Pubmed|19690372]

Expression and Regulation

Operons

• genes: [gene|42D2F9B958F62DFE447A1321E4109D676ECB899C|mtnW]-[gene|91633E6AF9A715166AE05BD7E540463DE352AA20|mtnX]-[gene|337F1E27C2E380DF8DCADE3581F74CFF52AFD224|mtnB]-[gene|C7D9E701B2CA2C9E85D69CAD6E0A45BE590BAED9|mtnD]
  description: [Pubmed|12022921]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12022921], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [regulon|S-box|S-box]: termination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination [Pubmed|10094622], in [regulon|S-box|S-box]

  regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]

References

• 10094622,19279009,12022921,14551435,11914366,12107147,19194007,15102328,19690372,12787499,18039762