tlpC [2020-04-07 17:41:31]

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein

locus: BSU_03440

pI: 4.93

mw: 61.62 kDa

protein length: 573 aa

gene length: 1722 bp

function: control of chemotaxis

product: methyl-accepting chemotaxis protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.4|Membrane-bound chemoreceptors]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 372,771 374,492

The protein

[SW|Domains]

• [SW|HAMP domain] (aa 194-246) (according to UniProt)
• [SW|Methyl-accepting transducer domain] (aa 280-544) (according to UniProt)

Structure

• [PDB|6S1K] (from E. coli, corresponds to aa 151 ... 498, 28.6% identity)

[SW|Localization]

• cell membrane [Pubmed|21515776]

Expression and Regulation

Operons

• genes: [gene|426253699CA4183F2E1023169C3B80AFA15108E4|tlpC]
  description: [Pubmed|7921238,7746143]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|7921238], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  additional information

  • in minimal medium,TlpC is present with 5,400 /- 1700 molecules per cell [PubMed|21515776]

Biological materials

Mutant

• BKE03440 ([gene|426253699CA4183F2E1023169C3B80AFA15108E4|tlpC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCCTCCCTTTACGA, downstream forward: _UP4_TGATGACTTACTATCATTAT
• BKK03440 ([gene|426253699CA4183F2E1023169C3B80AFA15108E4|tlpC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATTCCTCCCTTTACGA, downstream forward: _UP4_TGATGACTTACTATCATTAT

References

• 7921238,7746143,21515776,27899502