fliK

flagellar hook-length control, substrate specificity switch regulator

Locus: BSU_16270

Isoelectric point: 4.71

Molecular weight: 47.35 kDa

Protein length: 487 aa

Gene length: 1464 bp

Function: movement and chemotaxis

Product: flagellar hook-length control

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Flagellar proteins

Gene

Coordinates: 1,698,278 1,699,741

Phenotypes of a mutant

• inactivation of fliK confers resistance to high concentrations of Zn(II) PubMed
• no secretion of FlgM, permanent inhibition of SigD PubMed

The protein

Protein family

• FliK family (single member, according to UniProt)

Localization

• flagellum hook (according to UniProt)

Expression and Regulation

Operons

• Genes: ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • CodY: repression, in CodY regulon
  • DegU: regulation, in DegU regulon
  • SwrA: activation, in SwrA regulon

  Regulation

  • see fla-che operon

• Genes: flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliZ-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon

  Regulatory mechanism

  • Spo0A: repression, in Spo0A regulon
  • SwrA: activation, in SwrA regulon
  • CodY: repression, in CodY regulon
  • DegU: repression, in DegU regulon

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

Biological materials

Mutant

• BKE16270 (fliK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACCTTTCATTCCCCCTATT, downstream forward: _UP4_GAATGACTTCTATAAGTTCA
• BKK16270 (fliK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACCTTTCATTCCCCCTATT, downstream forward: _UP4_GAATGACTTCTATAAGTTCA

References

Reviews

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Original publications

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